Journal: Foods (Basel, Switzerland)

Article Title: Hericium erinaceus Protein Alleviates High-Fat Diet-Induced Hepatic Lipid Accumulation and Oxidative Stress In Vivo.

doi: 10.3390/foods14030459

Figure Lengend Snippet: Figure 6. Effect of HEP on the mRNA expression of hepatic lipid metabolism-related genes in obese mice: (A) PPARα (fold); (B) LPL (fold); (C) CPT-1a (fold); (D) ACOX1 (fold); (E) SREBP-1c (fold); (F) ACC1 (fold); (G) SCD-1 (fold); and (H) FASN (fold). The different lowercase letters (a, b, c, d) indicate significant differences between values in different groups (p < 0.05).

Article Snippet: Primary antibodies PPARα, CPT-1a, ACOX1, SREBP-1c, SCD-1, and FASN were purchased from Proteintech (Wuhan, China).

Techniques: Expressing

Journal: Foods (Basel, Switzerland)

Article Title: Hericium erinaceus Protein Alleviates High-Fat Diet-Induced Hepatic Lipid Accumulation and Oxidative Stress In Vivo.

doi: 10.3390/foods14030459

Figure Lengend Snippet: Figure 7. Effects of HEP on the protein expression of hepatic lipid metabolism-related genes in obese mice: (A) Representative images of Western blot; (B) PPARα (fold); (C) CPT-1a (fold); (D) ACOX1 (fold); (E) SREBP-1c (fold); (F) SCD-1 (fold); and (G) FASN (fold). The different lowercase letters (a, b, c, d) indicate significant differences between values in different groups (p < 0.05).

Article Snippet: Primary antibodies PPARα, CPT-1a, ACOX1, SREBP-1c, SCD-1, and FASN were purchased from Proteintech (Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Marine Drugs

Article Title: Effect of Marine Microalga Chlorella pyrenoidosa Ethanol Extract on Lipid Metabolism and Gut Microbiota Composition in High-Fat Diet-Fed Rats

doi: 10.3390/md16120498

Figure Lengend Snippet: Effect of CPE55 on the mRNA and protein expressions levels in the liver. The mRNA expression ( A ) and protein expression ( B , C ) of Acetyl CoA carboxylase (ACC), AMPK-α, 3-Hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA), and Sterol regulatory element-binding transcription factor-1c (SREBP-1c) levels were determined through real-time quantitative PCR (RT-qPCR) and western blotting analysis. Data are expressed as the mean ± SD. One-way ANOVA with Tukey’s test. * p < 0.05 and ** p < 0.01 for CPE55 versus NFD; # p < 0.05, ## p < 0.01 for CPE55 versus HFD.

Article Snippet: The membranes were incubated for 3.5 h at 37 °C using rabbit polyclonal antibodies against GAPDH, HMG-CoA, SREBP-1c, ACC, and AMPK-α (1:1000; Beyotime Biotechnology, Shanghai, China).

Techniques: Expressing, Binding Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

Journal: PLoS ONE

Article Title: Dimethylfumarate Suppresses Adipogenic Differentiation in 3T3-L1 Preadipocytes through Inhibition of STAT3 Activity

doi: 10.1371/journal.pone.0061411

Figure Lengend Snippet: Two-day post-confluent 3T3-L1 preadipocytes were subjected to adipogenic differentiation for 8 days in the absence or presence of various concentrations of DMF. (A) Real-time PCR was used to quantify the effect of DMF on adipogenic gene expression. On day 8, adipogenic markers were analyzed by real-time PCR using mRNA isolated from DMF-treated 3T3-L1 cells. (B) Representative Western blot analysis showing the effect of DMF on C/EBPβ, C/EBPα, PPARγ, and SREBP-1c levels. Data are presented as mean ± S.D. (n = 3); *p<0.05, **p<0.01, ***p<0.001 vs. control.

Article Snippet: The antibodies used in this study were directed against SREBP-1c, p21, p27 (BD Biosciences, San Jose, CA, USA), FAS, C/EBPα, PPARγ, Cyclin D, pRb, STAT3, phospho-STAT3 (Cell Signaling, Beverly, MA, USA), C/EBPβ (Santa Cruz Biotechnology, CA, USA).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Isolation, Western Blot

Journal: PLoS ONE

Article Title: Dimethylfumarate Suppresses Adipogenic Differentiation in 3T3-L1 Preadipocytes through Inhibition of STAT3 Activity

doi: 10.1371/journal.pone.0061411

Figure Lengend Snippet: 3T3-L1 cells were treated with 75 µM DMF for the indicated time periods during 3T3-L1 preadipocyte differentiation. (A) Schematic model depicting DMF treatment during 3T3-L1 preadipocyte differentiation. Thick lines indicate the treatment time at 75 µM DMF. 3T3-L1 cells were treated with 75 µM DMF for the indicated times during 3T3-L1 preadipocyte differentiation. (B) Representative micrographs showing cell culture dishes and cell monolayers stained with Oil Red O (× 200). (C) Quantitative analysis of Oil Red O staining. Oil Red O stained cells were extracted with isopropyl alcohol and the absorbance at 500 nm was measured. (D) Western blot analysis showing the protein levels of C/EBPβ, C/EBPα, PPARγ, SREBP-1c (precursor and mature), and FAS in 3T3-L1 cells after 0, 2, or 4 days of DMF treatment. The data are presented as mean ± S.D. (n = 3); *p<0.05, **p<0.001 vs. control.

Article Snippet: The antibodies used in this study were directed against SREBP-1c, p21, p27 (BD Biosciences, San Jose, CA, USA), FAS, C/EBPα, PPARγ, Cyclin D, pRb, STAT3, phospho-STAT3 (Cell Signaling, Beverly, MA, USA), C/EBPβ (Santa Cruz Biotechnology, CA, USA).

Techniques: Cell Culture, Staining, Western Blot

Journal: PLoS ONE

Article Title: Dimethylfumarate Suppresses Adipogenic Differentiation in 3T3-L1 Preadipocytes through Inhibition of STAT3 Activity

doi: 10.1371/journal.pone.0061411

Figure Lengend Snippet: Primer sequences for RT-PCR and Real-time PCR.

Article Snippet: The antibodies used in this study were directed against SREBP-1c, p21, p27 (BD Biosciences, San Jose, CA, USA), FAS, C/EBPα, PPARγ, Cyclin D, pRb, STAT3, phospho-STAT3 (Cell Signaling, Beverly, MA, USA), C/EBPβ (Santa Cruz Biotechnology, CA, USA).

Techniques: Real-time Polymerase Chain Reaction